Glycan-related products - ENGase

About ENGases

Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides.
In addition, some ENGases have transglycosylation activity, and can transfer sugar chains to sugar or glycoconjugate as acceptor.

Endo-CC is an ENGase from the basidiomycete fungus Coprinopsis cinerea, and catalyzes hydrolysis of N-linked oligosaccharides, especially high-mannose type and biantennary complex type.
Endo-CC N180H is Endo-CC mutant, and can transfer sugar chains for example SG-Oxazoline (product number:171810) as donor to GlcNAc as acceptor. 

Reaction Example:

 
TRANSGLYCOSYLATION PEPTIDES/ PROTEINS

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Products

Catalog Number	Product Name	Size	Source	Unit Definition
FSP-171831	Endo-CC (Wild Type)	300 munits	Endo-CC recombinant from Coprinopsis cinerea is expressed in E.coli as a fusion to His tag.	One unit is defined as the amount of enzyme that produce 1μmol SG from SGP per minute at 37℃, pH 8.0.
FSP-171832	Endo-CC N180H (Mutant)	300 munits	Endo-CC N180H recombinant from Coprinopsis cinerea is expressed in E.coli as a fusion to His tag.	One unit is defined as the amount of enzyme that produce 1μmol SG-GlcNAc-pNP from pNP-GlcNAc per minute at 30˚C, pH7.5.

Publications

1) Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K:
Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea.
PloS one 10, e0132859 (2015)

2) Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K:
Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.
Biotechnol. Lett. 39, 157-162 (2017)

3) Manabe S, Yamaguchi Y, Abe J, Matsumoto K, Ito Y:
Acceptor range of endo-β-N-acetylglucosaminidase mutant endo-CC N180H:from monosaccharide to antibody.
R Soc Open Sci, 5 (5) , 171521 2018 16 eCollection May (2018)