Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macro-molecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology.
The most commonly used method of separating suspensions of mixed populations of biological particles is centrifugation in density gradients - and the choice of medium used to form the gradients is critical for the retention of particles function. Since the early sixties Nycomed have been developing and producing iodinated compounds as X-ray imaging agents. It was recognized early on that these compounds were also well suited to the formation of density gradients and offered new opportunities for improved methods of separating biological particles, especially osmotically-sensitive cells and subcellular organelles. Nycomed has therefore been actively involved over the last 30 years in the research and development of density gradient media.
| Product | Description | Product Documents | |
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OptiPrep™
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The Optimal Density Gradient MediaOptiPrep is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance. |
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Nycodenz®
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A Universal Density Gradient MediaNycodenz® is an off-white powder, freely soluble in water. Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared. |
Nycodenz™ Leaflet |  |
Lymphoprep™Sodium diatrizoate: 9.1% (w/v) |
Ready-Made Solution for Isolating Human Mononuclear Cells (PBMC). GMP compliant and ISO 13485 certified.Lymphohoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human mononuclear cells. |
Lymphoprep™ Leaflet |  |
Lymphoprep Tube™ |
Convenient Fritted Centrifuge Tubes Prefilled with Lymphoprep™.
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Lymphoprep™ Tube Leaflet
Lymphoprep™ Tube Package Insert
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Polymorphprep™Sodium diatrizoate: 13.8% (w/v).Dextran 500: 8.0% (w/v). Density: 1.113 ± 0.001 g/ml. Osmolality: 445 ± 15 mOsm. Endotoxins: < 1.0 EU/ml. |
Ready-Made Solution For Isolation of Human Polymorphonuclear CellsPolymorphprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes). |
Polymorphprep™ Leaflet |  |
Polysucrose™ 400 |
Versatile Non-Ionic, Hydrophillic, Water-Soluble Powder for Density Gradient Separations And Other ApplicationsPolysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. The product is similar to Ficoll® 400 from GE Healthcare. |
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new content
GenomONE™ HVJ-Envelope Kits for
Cell Fusion and Truly Unique Transfection.
Transfection by membrane fusion? GenomONE™ delivers. Experienced transfectors know that developing an optimal protocol for transfecting a given cell/cargo pair is an empirical process. GenomONE's transfection by cell fusion kits are an efficient and totally unique alternative for your transfection toolbox. If you haven’t transfected with GenomONE™ you haven't transfected with anything like it.
GenomONE™ series products from Ishihara Sangyo KK (Japan) repurpose the membrane fusion ability of inactivated Sendai virus (aka Hemagglutinating Virus of Japan (HVJ) for laboratory use. GenomONE™-Si EX, GenomONE™-GX EX, and GenomeONE™-Neo EX kits are purposed specifically for transfection. GenomONE™-CF EX is purposed for cell fusion. Both kit types contain all essential reagents and buffers for use.
Inactivated, purified HVJ particles (HVJ-Envelope, HVJ-E) are non-infectious vesicles ~300 nm in diameter that retain the viral HN and F surface proteins involved in cell recognition and membrane fusion, respectively. HN protein attaches HVJ-E to sialic acid-containing cell receptors found on almost all animal cells. Binding of HN protein to the receptor induces a conformational change allowing the F protein to trigger membrane fusion.
Permeabilized HVJ-E vesicles readily incorporate molecular cargo. Treatment of cells or tissue with cargo-loaded HVJ-E vesicles results in membrane fusion and cargo delivery directly into the cell cytoplasm.
In contrast, molecular cargo introduced with conventional cationic-lipid based transfection reagents enter cells via endocytosis and may therefore be damaged by lysosomal activity.
- GenomeOne Dashboard
- GenomONE™ Series Products
- NEW! GenomeONE™-GE
(Cas9protein/gRNA transfection) - GenomeONE™-Si
(siRNA/mRNA transfection) - GenomeONE™-GX
(in vitro plasmid
transfection) - GenomONE™ Neo
(in vivo + in vitro transfection) - GenomONE™-CF fusion reagent
- GenomeONE™ Series Documents and Resources
- GenomOne Series Catalog
- Foundational Reference
Kaneda et al., Mol Ther 2002 - Literature Citations
- Transfection: in vivo
- Transfection: in vitro
- Cell and Membrane Fusion
- Manufacturer's Site
- GenomONE Series homepage
GenomONE™ Series Products
GenomONE™-GE EX: HVJ-E Cas9/gRNA Transfection Kit
- Applicable to CRISPER-CAS9-mediated knockout and knockin.
- GenomONE-GE EX enables efficient Cas9 protein/gRNA transfection in a variety of difficult-to-transfect cells.
- Applicable for difficult-to-transfect cells (e.g., Mouse primary T cell, U-937, Jurkat)
- Validation data for 23 cell types.
- Easy to use with simple and fast 5-10 minute protocol
- (note: For plasmid DNA transfection for Cas9 and gRNA expression, please use GenomONE-GX EX.)
GenomONE™-Si EX: HVJ-E transfection kit for small RNA transfection
- Optimized for transfection of synthetic siRNA/miRNA.
- Applicable to difficult to transfect immune cells in suspension like Raji, THP-1, U-937, Jurkat, HL-60, mouse primary Tcells)
- Easy to use with simple and fast 5-10 minute protocol
- Suitable for high-throughput procedures
- Suitable for in vivo (whole animal) transfection.
GenomONE™-GX EX: HVJ-E transfection kit for plasmid DNA
- Optimized for DNA delivery.
- High efficiency with difficult to transfect cells such as Jurkat, K-562, RAW 264.7, THP-1 compared to lipofection.
- Contains transfection enhancer to suppress cellular DNA degradation pathways.
- Transfection efficiency further enhanced with KALA peptide (not included)
- Suitable for high-throughput procedures.
- Suitable for in vivo (whole animal) transfection.
- Optimal conditions determined for 28 common cell lines.
GenomONE™-Neo EX: HVJ-E transfection kit for versatile cargo delivery in vitro and in vivo
- Versatile reagent for DNA, siRNA/miRNA, protein transfection
- The orginal GenomONE transfection reagent.
- Applicable for in vivo (whole animal) transfection.
GenomONE™-CF EX: potent HVJ-E cell fusion reagent
- Replaces PEG-based or electrofusion methods.
- High efficiency for hybridoma development.
- Suitable for adherent cells and cells grown in suspension.
- Highly cited in the literature for a variety of uses.
| GenomONE Series Product Selection Guide | ||||||
| use | cargo | GenomONE-GE | GenomONE-Si | GenomONE-GX | GenomONE-Neo | GenomONE-CF |
| in vitro transfection | Cas9 protein + gRNA | +++ | + | -- | + | -- |
| plasmid DNA | -- | -- | +++ | + | -- | |
| siRNA, miRNA | -- | +++ | -- | + | -- | |
| Protein | -- | + | -- | +++ | -- | |
| in vivo transfection | -- | + (siRNA, miRNA) |
-- | +++ | -- | |
| cell/membrane fusion | -- | -- | -- | -- | +++ | |
| ++: highly recommended; +: applicable but not ideal; --: not recommended | ||||||
Kaneda, Y., et al.: Hemagglutinating virus of Japan（HVJ）envelope vector as a versatile gene delivery system. Molecular Therapy, 6, 219-226（2002）
The HVJ genomic RNA of all GenomONE series products is completely inactivated for safe and easy use.
GenomONE™ is a trademark of the Ishihara Sangyo Corporation. 2021. All rights reserved.