- CAC Antibody Collection Index Page
Antibody Group
Advanced glycation end products (AGEs)
Autophagy and apoptosis
Bacteria-related
Calcium-binding proteins
Cancer
CD44 for enriching cancer stem cells
Tumor markers
Tumor inhibitors
Chaperones
Cytoskeleton
DNA damage
UV-induced DNA lesions
8-Nitroguanosine for oxidative stress research
Nucleotide excision repair factors
Epigenetics and chromatin
Histone H3 variants
Post-translationally-modified histone H3
Chromatin structure modifiers
Drosophila chromatin
Epitope tags
Exosomes
Extracellular matrix
Glycosaminoglycans (GAGs)
Proteoglycans
Matrix and basement membrane
Cell adhesion and hemidesmosome-related
Bone and cartilage-related
Wound repair-related
Hedgehog pathway
Hormones
Immunology
Fish CD4 and CD8α
Allergic disease-related
Adaptive and innate immunity
Macrophages
Inflammatory cytokines
Viral recognition pathways
Vpr for HIV research
Insulin-like growth factor-related
Mitochondria-related
Neurobiology
Neurodegenerative disease markers
Muscarinic acetylcholine receptors
Miscellaneous
Nuclear import and export
Oxidative stress
Plant-related
Plant hormones
Plant autophagy and apoptosis
Plant stress response
Plant stress response
Proteasomes
Puromycin-specific
Reproductive biology
Small molecules
Stem cells
Novel iPS/ES markers
Pluripotency-associated
Sumoylation pathway
TGF-beta pathway
TGF-beta LAP-d
TGF-beta signaling
Transcription factors
Transporters
Tyrosine phosphatases
Ubiquitin-Proteasome Related
CAC Antibody Collection
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Puromycin-specific
Puromycin is an aminonucleoside antibiotic derived from the bacterial strain Streptomyces alboniger. Structurally resembling the 3' end of aminoacyl-tRNAs, it can bind to translating ribosomes and cause premature chain termination. Classically, protein biosynthesis has been monitored using pulse-chase and flooding dose methods which both employ radioactive amino acid labels.Product name (click for order info) | Cat No (click for datasheet) |
Host | Species specificity |
Anti Puromycin mAb (Clone 3RH11) | CAC-PEN-MA001 | MS | - |
Product name | Anti Puromycin mAb (Clone 3RH11) |
Cat No | CAC-PEN-MA001 |
Description | Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling and allows for the direct evaluation/quantification of translation using standard immunochemical methods. Features 1) Easy to evaluate and quantify protein biosynthesis 2) An alternative to the traditional pulse-chase method that relies on radioactive amino acids 3) Applicable to Western blot and ELISA analyses References: 1) Kelleher AR, Gordon BS, Kimball SR, Jefferson LS. Changes in REDD1, REDD2, and atrogene mRNA expression are prevented in skeletal muscle fixed in a stretched position during hindlimb immobilization. Physiol Rep. 2014 Feb 25;2(2):e00246. doi: 10.1002/phy2.246. eCollection 2014 Feb 1. PubMed PMID: 24744910; PubMed Central PMCID: PMC3966240. 2) Kelleher AR, Kimball SR, Dennis MD, Schilder RJ, Jefferson LS. The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb. Am J Physiol Endocrinol Metab. 2013 Jan 15;304(2):E229-36. doi: 10.1152/ajpendo.00409.2012. Epub 2012 Nov 27. PubMed PMID: 23193052; PubMed Central PMCID: PMC3543567. |
Host | Mouse |
Species specificity | - |
Figure 1 | ![]() |
Immunoblot Human fibroblasts (TIG-1 cells) were plated in 12-well plates and cultured till 80% confluent. Puromycin (final conc. 0 uM and 5 uM) was added to the medium of the cells 80 min before harvest. Wells were washed with PBS, followed by addition of 1mL buffer (20 mM Tris-HCl (pH8.0) + protease inhibitor) and then ultrasonic fragmentation. Lysate was centrifuged (4°C, 12,000 g, 20 min) and supernatant was collected for downstream assays (Western Blotting, ELISA). Loaded 15 ug/lane. Primary Antibody (anti puromycin [CAC-PEN-MA001]) 1:1,000 dilution (1 ug/mL). Secondary Antibody (anti mouse Ig-HRP, DAKO, Cat.No. P0161)1:2,000 dilution |