Cell / Cell culture products

 Bone marrow origin (BMSC)

Bone marrow-derived mesenchymal stem (stromal) cells (product number: MSB01C) are cells obtained by passage (passage 1) the BMSC fraction from a cell group isolated from SD rat bone marrow. Please use as a set with dedicated growth medium and supplements for medium addition.

Background

Mesenchymal stem cells (MSC), a kind of somatic stem cells, can be isolated from adipose tissue, bone marrow, umbilical cord, dental pulp, etc. In 2006, Dominici et al. reported that human mesenchymal stem cells (MSCs) (1) adhere to plastic culture vessels, (2) CD105, CD73, CD90 as positive markers, CD45, CD34, CD14, CD11b, CD79a, CD19, HLA - Class II (DR) as a negative marker, and (3) ability to differentiate into bone, fat, and cartilage. In addition to their excellent self-proliferative properties, MSCs have the ability to differentiate into not only bone, cartilage, and fat, but also liver, etc. They do not express MHC Class-II, have immunoregulatory functions, accumulate at diseased sites, and act in various ways. It is known to have excellent characteristics such as the secretion of cytokines and growth factors and the effect of promoting tissue regeneration and repair.

 

Composition

Product name	animal	capacity	Catalog number
Rat bone marrow-derived mesenchymal stem cells (BMSC)	SD rat	0.5×10 ^6 cells 1 piece	PMC-MSB01C
Mouse subcutaneous fat-derived mesenchymal stem cells (stromal cells)	ICR mouse	1×10 ^6 cells 1 piece	PMC-MSA11C

 Frozen Cells  : The cells are delivered frozen in dry ice packaging. You can take it out immediately after delivery and start culturing immediately, or you can freeze it at the temperature specified in the instruction manual.

 

Culture example

Figure 1. Rat subcutaneous adipose-derived mesenchymal stem cells 5 days after culture	Figure 2. Staining of adipose-differentiated rat subcutaneous adipose-derived mesenchymal stem cells with a lipid assay kit	Figure 3. Cartilage-differentiated rat bone marrow-derived mesenchymal stem cells stained with alcian blue	Figure 4. Mouse mesenchymal stem cells derived from subcutaneous fat, day 4 of culture
Figure 5. Adipogenic mouse subcutaneous adipose-derived mesenchymal stem cells stained with Lipid Assay Kit	Figure 6. Alcian blue staining of cartilage-differentiated mouse subcutaneous adipose-derived mesenchymal stem cells	Figure 7. Staining osteogenic mouse subcutaneous adipose-derived mesenchymal stem cells with a mineralization staining kit

(click image to enlarge)