CD44 variant antibodies and kit for characterizing and isolating cancer stem cells : 

Application examples

 

Application examples of CD44 variant antibodies for cancer stem cell detection

■ Application example 1. 

In vitro evaluation system for cancer stem cell (CSC) therapeutic drugs based on sphere-forming ability

The Sphere Formation Assay is a representative in vitro system for evaluating the effect of drugs on cancer stem cells (CSCs). This is a method for quantifying the number of CSCs by the number of colonies (called spheres) formed in a culture system, utilizing the tumor-forming ability that is a characteristic of CSCs. This makes it possible to quantify the effect of a drug on CSCs by the reduction in the number of spheres when the drug is applied.

Sphere formation by prostate cancer cell line PC-3

■ Application Example 2. 

In vivo evaluation system for cancer stem cell (CSC) therapeutic drugs based on metastasis formation ability

Cancer stem cells (CSCs) have the ability to form metastatic lesions in distant organs.

When a highly enriched group of cancer stem cells (CSCs) enriched using this antibody is injected into the tail vein, a significantly large number of lung metastatic lesions are formed, making it possible to confirm the effectiveness of drug administration.

Metastatic formation by tail vein injection of highly enriched cancer stem cells (CSCs) from pancreatic cancer cell line AsPC-1

The high efficiency of metastasis (colony) formation by CD44 v9-High cells presents an opportunity to assay the effectiveness of new anti-CSC therapeutic strategies.

Ordering Information

Name	Catalog Number	Host	Reactivity	Clone	Applications
Anti CD44 v10-e16	CAC-LKG-M002 CAC-LKG-M002S	RT	MS	RM1	FC IHC
Anti CD44 v9	CAC-LKG-M001 CAC-LKG-M003	RT	HU	RV3	FC WB IHC(p) IF ELISA IP MACS Photo-immunotherapy
Magnetic Cell separation Kit for Human CD44v9+ Cancer Stem Cell	CSR-CSC-SEP1	RT	HU	RV3	Cancer stem cell isolation