TRAP Staining Kit

For staining of Tartrate-Resistant Acid Phosphatase in osteoclasts

The TRAP Staining Kit is used for the staining of Tartrate-Resistant Acid Phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and the activity of osteoclasts.

Components

• ●Tartrate-containing Buffer
• ●Chromogenic Substrate

One kit contains regents for 10 × 96-well plates

Data

Figure 1. TRAP staining of Osteoclast

Figure 2. TRAP staining of Rat Osteoclast

Citations

1. A Novel Therapeutic Peptide as a Partial Agonist of RANKL in Ischemic Stroke. Sci Rep. 2016 Nov 29;6:38062. doi: 10.1038/srep38062.
2. Ecklonia cava Extract Containing Dieckol Suppresses RANKL-Induced Osteoclastogenesis via MAP Kinase/NF-κB Pathway Inhibition and Heme Oxygenase-1 Induction. J Microbiol Biotechnol. 2019 Jan 28;29(1):11-20. doi: 10.4014/jmb.1810.10005.
3. Anti-receptor activator of nuclear factor κB ligand antibody treatment increases osteoclastogenesis-promoting IL-8 in patients with rheumatoid arthritis. Int Immunol. 2019 Apr 26;31(5):277-285. doi: 10.1093/intimm/dxz009.
4. Sema6A-plexin-A2 axis stimulates RANKL-induced osteoclastogenesis through PLCγ-mediated NFATc1 activation. Life Sci. 2019 Apr 1;222:29-35. doi: 10.1016/j.lfs.2019.01.060. Epub 2019 Feb 28.
5. The pivotal role of microRNA-21 in osteoclastogenesis inhibition by anthracycline glycoside aloin. J Nat Med. 2019 Jan;73(1):59-66. doi: 10.1007/s11418-018-1237-3. Epub 2018 Aug 16.
6. Bruton's tyrosine kinase (Btk) inhibitor tirabrutinib suppresses osteoclastic bone resorption. Bone Rep. 2019 Mar 15;10:100201. doi: 10.1016/j.bonr.2019.100201. eCollection 2019 Jun.
7. Conditional Controlled Light/Dark Cycle Influences Exercise-Induced Benefits in a Rat Model with Osteoarthritis: In Vitro and In Vivo Study. J Clin Med. 2019 Nov 2;8(11):1855. doi: 10.3390/jcm8111855.
8. The combined effects of teriparatide and anti-RANKL monoclonal antibody on bone defect regeneration in ovariectomized mice. Bone. 2020 Jan;130:115077. doi: 10.1016/j.bone.2019.115077. Epub 2019 Oct 14.
9. Effects of single or combination therapy of teriparatide and anti-RANKL monoclonal antibody on bone defect regeneration in mice. Bone. 2018 Jan;106:1-10. doi: 10.1016/j.bone.2017.09.021. Epub 2017 Oct 1.
10. In Vitro Effects of Weissella cibaria CMU and CMS1 on Receptor Activator of NF-κB Ligand (RANKL)-Induced Osteoclast Differentiation. J Funct Biomater. 2024 Mar 8;15(3):65. doi: 10.3390/jfb15030065.

More

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12. Effect of calcium silicate-based endodontic sealer on the retention of fiber posts cemented at different time intervals. Saudi Dent J. 2021 Nov;33(7):718-723. doi: 10.1016/j.sdentj.2020.04.002. Epub 2020 Apr 10.
13. Extracellular microRNAs induce dendritic cell-dependent joint inflammation and potentiate osteoclast differentiation via TLR7/8 engagement. J Autoimmun. 2024 May;145:103189. doi: 10.1016/j.jaut.2024.103189. Epub 2024 Mar 4.
14. Conditioned-medium of stem cells from human exfoliated deciduous teeth prevent apoptosis of neural progenitors. Saudi Dent J. 2022 Nov;34(7):565-571. doi: 10.1016/j.sdentj.2022.08.005. Epub 2022 Aug 23.
15. Iguratimod suppresses sclerostin and receptor activator of NF-κB ligand production via the extracellular signal-regulated kinase/early growth response protein 1/tumor necrosis factor alpha pathway in osteocytes and ameliorates disuse osteoporosis in mice. Bone. 2024 Apr;181:117026. doi: 10.1016/j.bone.2024.117026. Epub 2024 Feb 5.
16. S-nitrosoglutathione reductase-dependent p65 denitrosation promotes osteoclastogenesis by facilitating recruitment of p65 to NFATc1 promoter. Bone. 2024 Apr;181:117036. doi: 10.1016/j.bone.2024.117036. Epub 2024 Feb 2.
17. EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy. iScience. 2024 Jan 11;27(2):108871. doi: 10.1016/j.isci.2024.108871. eCollection 2024 Feb 16.
18. Innervation and osteoclast distribution in the inferior pharyngeal jaw of the cichlid Nile tilapia (Oreochromis niloticus). Anat Rec (Hoboken). 2024 Jun;307(6):2139-2148. doi: 10.1002/ar.25381. Epub 2024 Jan 6.
19. A novel macrolide-Del-1 axis to regenerate bone in old age. iScience. 2024 Jan 4;27(2):108798. doi: 10.1016/j.isci.2024.108798. eCollection 2024 Feb 16.
20. Lateral bone ridge expansion and internal tissue replacement for vertebral body growth in Pacific bluefin tuna Thunnus orientalis. J Morphol. 2024 Feb;285(2):e21666. doi: 10.1002/jmor.21666.
21. Attenuative effects of collagen peptide from milkfish (Chanos chanos) scales on ovariectomy-induced osteoporosis. Food Sci Nutr. 2023 Oct 8;12(1):116-130. doi: 10.1002/fsn3.3746. eCollection 2024 Jan.
22. Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclasts. Cell Struct Funct. 2023 Nov 17;48(2):223-239. doi: 10.1247/csf.23061. Epub 2023 Nov 10.
23. Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination. Cell Death Dis. 2023 Aug 30;14(8):576. doi: 10.1038/s41419-023-06101-7.
24. Ixeris dentata and Lactobacillus gasseri media protect against periodontitis through Nrf2-HO-1 signalling pathway. Sci Rep. 2023 Aug 8;13(1):12861. doi: 10.1038/s41598-023-39853-5.
25. Osteoclastogenesis Requires Primary Cilia Disassembly and Can Be Inhibited by Promoting Primary Cilia Formation Pharmacologically. Cells Tissues Organs. 2024;213(3):235-244. doi: 10.1159/000531098. Epub 2023 May 22.
26. Osteoblast-derived extracellular vesicles exert osteoblastic and tumor-suppressive functions via SERPINA3 and LCN2 in prostate cancer. Mol Oncol. 2023 Oct;17(10):2147-2167. doi: 10.1002/1878-0261.13484. Epub 2023 Aug 4.
27. Effect of Ishophloroglucin A Isolated from Ishige okamurae on In Vitro Osteoclastogenesis and Osteoblastogenesis. Mar Drugs. 2023 Jun 26;21(7):377. doi: 10.3390/md21070377.
28. RANKL inhibition reduces lesional cellularity and Gα(s) variant expression and enables osteogenic maturation in fibrous dysplasia. Bone Res. 2024 Feb 20;12(1):10. doi: 10.1038/s41413-023-00311-7.
29. Eribulin mesylate induces bone mass loss by promoting osteoclastic bone resorption in mice. Bone Rep. 2023 May 30;18:101693. doi: 10.1016/j.bonr.2023.101693. eCollection 2023 Jun.
30. Effects of chondroitin sulfate oligosaccharides on osteoclast differentiation of RAW264 cells, and myotube differentiation of C2C12 cells. PLoS One. 2023 Apr 13;18(4):e0284343. doi: 10.1371/journal.pone.0284343. eCollection 2023.
31. Changes in superoxide dismutase 3 (SOD3) expression in periodontal tissue during orthodontic tooth movement of rat molars and the effect of SOD3 on in vitro hypoxia-exposed rat periodontal ligament cells. Eur J Orthod. 2023 Jul 31;45(4):430-437. doi: 10.1093/ejo/cjad005.
32. The Fungal Metabolite (+)-Terrein Abrogates Inflammatory Bone Resorption via the Suppression of TNF-α Production in a Ligature-Induced Periodontitis Mouse Model. J Fungi (Basel). 2023 Mar 3;9(3):314. doi: 10.3390/jof9030314.
33. Novel Bruton's tyrosine kinase inhibitor TAS5315 suppresses the progression of inflammation and joint destruction in rodent collagen-induced arthritis. PLoS One. 2023 Feb 23;18(2):e0282117. doi: 10.1371/journal.pone.0282117. eCollection 2023.
34. Dasatinib, a selective tyrosine kinase inhibitor, prevents joint destruction in rheumatoid arthritis animal model. Int J Rheum Dis. 2023 Apr;26(4):718-726. doi: 10.1111/1756-185X.14627. Epub 2023 Feb 21.
35. Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration. Elife. 2023 Feb 3;12:e80122. doi: 10.7554/eLife.80122.
36. Crucial Role of Lysine-Specific Histone Demethylase 1 in RANKL-Mediated Osteoclast Differentiation. Int J Mol Sci. 2023 Feb 10;24(4):3605. doi: 10.3390/ijms24043605.
37. Essentiality of Nfatc1 short isoform in osteoclast differentiation and its self-regulation. Sci Rep. 2023 Nov 1;13(1):18797. doi: 10.1038/s41598-023-45909-3.
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39. Extracellular Fe(2+) and Fe(3+) modulate osteocytic viability, expression of SOST, RANKL and FGF23, and fluid flow-induced YAP1 nuclear translocation. Sci Rep. 2023 Dec 1;13(1):21173. doi: 10.1038/s41598-023-48436-3.
40. Anti-NF-κB peptide derived from nuclear acidic protein attenuates ovariectomy-induced osteoporosis in mice. JCI Insight. 2023 Nov 22;8(22):e171962. doi: 10.1172/jci.insight.171962.
41. Exosomes from dental pulp cells attenuate bone loss in mouse experimental periodontitis. J Periodontal Res. 2022 Jan;57(1):162-172. doi: 10.1111/jre.12949. Epub 2021 Nov 26.
42. DJ-1 controls T cell differentiation and osteoclastogenesis in rheumatoid arthritis. Sci Rep. 2022 Jul 27;12(1):12767. doi: 10.1038/s41598-022-16285-1.
43. EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis. Arthritis Res Ther. 2022 Nov 17;24(1):254. doi: 10.1186/s13075-022-02941-4.
44. Mitigating RANKL-induced cholesterol overload in macrophages with β-cyclodextrin-threaded polyrotaxanes suppresses osteoclastogenesis. Biomater Sci. 2022 Sep 13;10(18):5230-5242. doi: 10.1039/d2bm00833e.
45. Deer Antler Reserve Mesenchyme Cell-Conditioned Medium Reduces the Destruction of Periodontitis in Mice. Stem Cells Dev. 2022 Dec;31(23-24):766-776. doi: 10.1089/scd.2022.0110. Epub 2022 Oct 18.
46. Investigation of bone invasion and underlying mechanisms of oral cancer using a cell line-derived xenograft model. Oncol Lett. 2022 Sep 13;24(5):382. doi: 10.3892/ol.2022.13502. eCollection 2022 Nov.
47. Zoledronic acid generates a spatiotemporal effect to attenuate osteoarthritis by inhibiting potential Wnt5a-associated abnormal subchondral bone resorption. PLoS One. 2022 Jul 28;17(7):e0271485. doi: 10.1371/journal.pone.0271485. eCollection 2022.
48. Identification of Novel Genes for Cell Fusion during Osteoclast Formation. Int J Mol Sci. 2022 Jun 8;23(12):6421. doi: 10.3390/ijms23126421.
49. ROS-responsive resveratrol-loaded cyclodextrin nanomicelles reduce inflammatory osteolysis. Colloids Surf B Biointerfaces. 2022 Nov;219:112819. doi: 10.1016/j.colsurfb.2022.112819. Epub 2022 Sep 1.
50. IL-27 Modulates the Cytokine Secretion in the T Cell-Osteoclast Crosstalk During HIV Infection. Front Immunol. 2022 Apr 5;13:818677. doi: 10.3389/fimmu.2022.818677. eCollection 2022.
51. Pentosan polysulfate regulates hepcidin 1-facilitated formation and function of osteoclast derived from canine bone marrow. PLoS One. 2022 Mar 17;17(3):e0265596. doi: 10.1371/journal.pone.0265596. eCollection 2022.
52. Effect of Gamma Irradiation on the Osteoinductivity of Demineralized Dentin Matrix for Allografts: A Preliminary Study. J Funct Biomater. 2022 Jan 31;13(1):14. doi: 10.3390/jfb13010014.
53. SOST gene suppression stimulates osteocyte Wnt/β-catenin signaling to prevent bone resorption and attenuates particle-induced osteolysis. J Mol Med (Berl). 2023 May;101(5):607-620. doi: 10.1007/s00109-023-02319-2. Epub 2023 May 1.
54. A Novel HDAC1-Selective Inhibitor Attenuates Autoimmune Arthritis by Inhibiting Inflammatory Cytokine Production. Biol Pharm Bull. 2022;45(9):1364-1372. doi: 10.1248/bpb.b22-00321.
55. Excessive osteoclast activation by osteoblast paracrine factor RANKL is a major cause of the abnormal long bone phenotype in Apert syndrome model mice. J Cell Physiol. 2022 Apr;237(4):2155-2168. doi: 10.1002/jcp.30682. Epub 2022 Jan 20.
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57. D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis. Sci Rep. 2022 May 20;12(1):8580. doi: 10.1038/s41598-022-12206-4.
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60. Improvement of Biological Effects of Root-Filling Materials for Primary Teeth by Incorporating Sodium Iodide. Molecules. 2022 May 4;27(9):2927. doi: 10.3390/molecules27092927.
61. Astaxanthin Protects against Hyperglycemia-Induced Oxidative and Inflammatory Damage to Bone Marrow and to Bone Marrow-Retained Stem Cells and Restores Normal Hematopoiesis in Streptozotocin-Induced Diabetic Mice. Antioxidants (Basel). 2022 Nov 23;11(12):2321. doi: 10.3390/antiox11122321.
62. Immunomodulatory Effect and Bone Homeostasis Regulation in Osteoblasts Differentiated from hADMSCs via the PD-1/PD-L1 Axis. Cells. 2022 Oct 7;11(19):3152. doi: 10.3390/cells11193152.
63. Attenuative effects of collagen peptide from milkfish (Chanos chanos) scales on ovariectomy-induced osteoporosis. Food Sci Nutr. 2023 Oct 8;12(1):116-130. doi: 10.1002/fsn3.3746. eCollection 2024 Jan.
64. Protocols for collecting mouse PDL cells and bone marrow cells, differentiation, and data analysis. STAR Protoc. 2024 Jun 26;5(3):103162. doi: 10.1016/j.xpro.2024.103162.
65. Extracellular microRNAs induce dendritic cell-dependent joint inflammation and potentiate osteoclast differentiation via TLR7/8 engagement. J Autoimmun. 2024 May;145:103189. doi: 10.1016/j.jaut.2024.103189. Epub 2024 Mar 4.
66. In Vitro Effects of Weissella cibaria CMU and CMS1 on Receptor Activator of NF-κB Ligand (RANKL)-Induced Osteoclast Differentiation. J Funct Biomater. 2024 Mar 8;15(3):65. doi: 10.3390/jfb15030065.

Product List

TRAP Staining Kit

Product name	Catalog Number
TRAP Staining Kit	PMC-AK04F