Osteoblasts

For research on regulatory mechanisms of osteoblast activity and osteogenesis

Bone metabolism is composed of balanced bone formation of osteoblasts and bone resorption of osteoclasts. Osteoblast from cranial bone contains frozen osteoblasts isolated from rat or mouse calvariae. Osteoblast from cranial bone is a useful to study osteoblast and osteogenesis.

Specification

Description	Strain	Age	Tissue	Size	Catalog number
Osteoblasts (Frozen Cells)	SD Rat	1 to 4 days	cranial bone	1 × 10^6 cells / vial	PMC-OBC02C
Osteoblasts (Frozen Cells)	ICR mouse	1 to 4 days	cranial bone	1 × 10^6 cells / vial	PMC-OBC12C

Data

Figure 1. Rat Osteoblasts

Figure 2. ALP staining of rat osteoblast

Figure 3. Calcified Nodule Staining of osteoblast after 28 days of incubation

Citations

1. Hisa, I., Inoue, Y., Hendy, G. N., Canaff, L., Kitazawa, R., Kitazawa, S., Komori, T., Sugimoto, T., Seino, S., Kaji, H. Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with The Bone Morphogenetic Protein-Runx2 Pathway. J. Biol. Chem. 286, 9787-9796 (2011), (PMID: 21239498)
2. Itoh, T., Takeda, S., Akao, Y.
   MicroRNA-208 Modulates BMP-2-stimulated Mouse Preosteoblast Differentiation by Directly Targeting V-ets Erythroblastosis Virus E26 Oncogene Homolog 1.
   J. Biol. Chem. 285, 27745-27752 (2010), (PMID:20576608)
3. Takenawa, T., Kanai, T., Kitamura, T., Yoshimura, Y., Sawa, Y., Iida, J.
   Expression and Dynamics of Podoplanin in Cultured Osteoblasts with Mechanostress and Mineralization Stimulus.
   Acta Histochem. Cytochem. 51, 41-52 (2018), (PMID:29622849)
4. Nakashima, Y., Nahar, S., Miyagi-Shiohira, C., Kinjo, T., Kobayashi, N., Kitamura, S., Saitoh, I., Watanabe, M., Fujita, J., Noguchi, H.
   Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice.
   Int. J. Mol. Sci. 20, pii: E2672 (2019), (PMID:31151297)
5. Kishikawa, A., Kitaura, H., Kimura, K., Ogawa, S., Qi, J., Shen, WR., Ohori, F., Noguchi, T., Marahleh, A., Nara, Y., Ichimura, A., Mizoguchi, I.
   Docosahexaenoic Acid Inhibits Inflammation-Induced Osteoclast Formation and Bone Resorption in vivo Through GPR120 by Inhibiting TNF-α Production in Macrophages and Directly Inhibiting Osteaoclast Formation.
   Front Endocrinol (Lausanne). 10, 157 (2019), (PMID:30949128)

Product List

Osteoblasts from cranial bone

Product name	Catalog Number
Osteoblasts from cranial bone (rat)	PMC-OBC02C
Osteoblasts from cranial bone (mouse)	PMC-OBC12C

Notes

When cells have been properly stored in liquid nitrogen and cultured according to instructions using recommended media and reagents, we guarantee against poor growth at the initiation of culture. For product support, please contact us (email: primarycell@cosmobio.co.jp). The warranty period is [within 6 months after receiving the product]. Warranty is not applicable if changes are made to the media or its usage, or if refrozen cells are utilized.

Optimized Medium

Osteoblast Culture Medium is optimized for in vitro culture of osteoblasts (PMC-OBC02C and PMC-OBC12C).

Product name	Catalog Number
Osteoblast Culture Medium	PMC-OBCM