Alkaline Phosphatase Staining Kit

For the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells.

The Alkaline Phosphatase Staining Kit is used for the staining of alkaline phosphatase in osteoblasts. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP).

Components

  • ●Substrate-containing Buffer
  • ●Chromogenic Substrate

One kit contains regents for 10 × 12-well plates

Data

Figure 1. ALP staining of 3T3-E1

Citations

  1. Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery.
    J Biomater Appl. 2016 Nov;31(5):661-673. doi: 10.1177/0885328216667633. Epub 2016 Sep 6. PMID: 27604088
  2. Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/β-catenin signaling.
    Acta Biomater. 2019 Sep 15;96:594-604. doi: 10.1016/j.actbio.2019.06.016. Epub 2019 Jun 15. PMID: 31212112
  3. EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy.
    iScience. 2024 Jan 11;27(2):108871. doi: 10.1016/j.isci.2024.108871. eCollection 2024 Feb 16. PMID: 38313054
  4. A novel macrolide-Del-1 axis to regenerate bone in old age.
    iScience. 2024 Jan 4;27(2):108798. doi: 10.1016/j.isci.2024.108798. eCollection 2024 Feb 16. PMID: 38261928
  5. Morphological study of tooth development in podoplanin-deficient mice.
    PLoS One. 2017 Feb 21;12(2):e0171912. doi: 10.1371/journal.pone.0171912. eCollection 2017. PMID: 28222099
  6. Unusual effects of a nanoporous gold substrate on cell adhesion and differentiation because of independent multi-branch signaling of focal adhesions.
    J Mater Sci Mater Med. 2023 Oct 26;34(11):54. doi: 10.1007/s10856-023-06760-0. PMID: 37884819

Product List

Alkaline Phosphatase Staining Kit

Product nameCatalog Number
Alkaline Phosphatase Staining Kit PMC-AK20