biothelis

 

Endocan, also known as endothelial cell-specific molecule (ESM-1), is encoded by a single non-polymorphic gene (esm1) and was originally discovered by Lassalle and collaborators in endothelial cells. Structurally, endocan is a dermatan sulfate proteoglycan of 50 kDa (165aa) that is freely circulating in blood. Endocan binds CD11a/CD18 integrin (also called LFA-1 for Leukocyte Function-associated Antigen1) on human leukocytes inhibiting consequently its binding to ICAM-1 and transendothelial migration. Moreover, endocan has been recently described as a biomarker of tip cells and neoangiogenesis. Endocan expression is upregulated by pro-inflammatory molecules such as tumor necrosis factor alpha, and pro-angiogenic molecules including TNF , IL-1, VEGF or FGF-2. Its synthesis and secretion decrease by activation of the endothelial PI3K pathway induced by a variety of mediators such as gamma interferon or insulin. Circulating endocan declines through an activated neutrophil-induced proteolytic process, generating a circulating cleaved form of 14 kDa. Endocan binds via its dermatan sulfate chain to hepatocyte growth factor/ scatter factor.

Endocan appears as a pertinent biomarker of endothelial dysfunction. Biothelis offers numerous Research Use Only products for laboratories studying the Endocan molecule. Endocan has been described as a biomarker of pulmonary endothelial dysfunction. Early variations of blood levels of Endocan have been shown to predict nosocomial pneumonia or acute respiratory failure depending on the clinical context: septic shock, polytrauma, thoracic surgery, mechanical ventilation, and COVID19 (De Freitas Caires N et al,, Crit Care, 2018, review).

Indeed, BIOTHELIS is now introducing the measurement of blood endocan as an in vitro diagnostic medical device in French university Hospitals like Lille and Paris. Additionally, Endocan has also been shown to be strongly associated with angiogenesis, particularly involved in tumor growth. Nevertheless, much remains to be done to fully understand the real functions of endocan, BIOTHELIS offers the best tools for exploring the functions of human and animal endocan in experimental designs. Biothelis antibodies are all purified by affinity chromatography in native conditions, tested for their activities, and stored without preservatives. 

 

A new clinical Concept

Endocan exhibits anti-inflammatory activity, acting as an inhibitor of the leukocyte integrin-dependent activation cascade LFA-1. By preventing the entry of leukocytes into the interstitial tissues, endocan reduces inflammation. Thus, a high level of endocan in acute or chronic inflammatory syndromes would protect against inflammation, and would have a good prognosis. Conversely, a low level of endocan in these syndromes would be a poor prognosis or predictor of a pathological event.

New clinical applications

Clinical research has established endocan as a diagnostic biomarker for the endothelial response that occurs in many diseases. IV administration of LPS in humans induces an increase in human plasma endocan which correlates with a defective vasodilator response to acetylcholine. An increase or decrease in endocan identifies patients at risk for pulmonary infection / aggression or tumor aggression.

The dosage of circulating endocan can be useful to the patient in different ways:

• Early diagnosis to allow earlier start of treatment (ARDS, hepatocarcinoma),
• Risk stratification (pneumonia),
• The prognosis (cirrhosis, oncology),
• The response to treatment (oncology),
• Reducing hospital costs.

Learn more about clinical applications

All products on this page are for research use only and not for dianostic or therapeutic use.  

ANTIBODIES

Name	Clone	Specificity	Reactivity	Application	Size	Cat. No.
Anti-Endocan mAb(Clone MEP08)	MEP08	N-termnal	Chicken, Monkey, Human	IHC	100 µg	BTL-LIA-0901
Anti-Endocan mAb (Clone MEP21)	MEP21	N-termnal	Human	WB	100 µg	BTL-LIA-0902
Anti-Endocan mAb (Clone GGR222)	GGR222	N-terminal	Mouse, Rat	WB	100 µg	BTL-LIA-0905
Anti-Endocan mAb (Clone MEP14)	MEP14	C-terminal	Human, Chicken, Rat, Porcine, Mouse, Monkey	IHC, ELISA, WB	100 µg	BTL-LIA-1001
Anti-Endocan mAb (Clone MEC15)	MEC15	N-termnal	Human, Monkey	ELISA, FC, WB	100 µg	BTL-LIA-1002
Anti-Endocan mAb (Clone MEP19)	MEP19	C-termnal	Human	IP	100 µg	BTL-LIA-1003
Anti-Endocan mAb (Clone GGR237)	GGR237	internal	Mouse, Rat	IP	100 µg	BTL-LIA-1101

 PROTEINS

Name	Clone	Reactivity	Application	Size	Cat. No.
Human Endocan/ESM-1 50 kDa (recombinant)			bioassay, western blot	10 µg	BTL-LIP-1001-10U
Human Endocan/ESM-1 50 kDa (recombinant)			bioassay, western blot	50 µg	BTL-LIP-1001-50U
Human Endocan/ESM-1 20 kDa (recombinant)			bioassay, western blot	10 µg	BTL-LIP-1002-10U
Human Endocan/ESM-1 20 kDa (recombinant)			bioassay, western blot	50 µg	BTL-LIP-1002-50U

 ELISA Development KITS

Name	Designation	Specificity	Description	Reactivity	Application	Size	Cat. No.
Human ESM-1 (DIYEK H1) ELISA Assay	DIYEK H1	Human Endocan/ESM-1 Monkey Endocan/ESM-1	To realize two 96 well plates	Human, Monkey	ELISA Development	2 x 96 rxns	BTL-LIK-1101
Human ESM-1 (DIYEK H1) ELISA Assay	DIYEK H1	Human Endocan/ESM-1	To realize five 96 well plates	Human, Monkey	ELISA Development	5 x 96 rxns	BTL-LIK-1101B5
Human ESM-1 (DIYEK H1) ELISA Assay	DIYEK H1	Human Endocan/ESM-1	To realize ten 96 well plates	Human, Monkey	ELISA Development	10 x 96 rxns	BTL-LIK-1101B10
Human ESM-1 (DIYEK H1) ELISA Assay	DIYEK H1	Human Endocan/ESM-1	To realize twenty-five 96 well plates	Human, Monkey	ELISA Development	25 x 96 rxns	BTL-LIK-1101B25
Mouse ESM-1 (DIYEK M1) ELISA Assay	DIYEK H1	Mouse Endocan/ESM-1	To realize two 96 well plates	Mouse	ELISA Development	2 x 96 rxns	BTL-LIK-1102
Rat ESM-1 (DIYEK M1) ELISA Assay	DIYEK H1	Rat Endocan/ESM-1	To realize two 96 well plates	Rat	ELISA Development	2 x 96 rxns	BTL-LIK-1103
Human ESM-1 (JDIEK H1) ELISA Assay	DIYEK H1	Human Endocan/ESM-1 Complete ELISA KIT	Ready to use ELISA Kit with all buffers and reagents	Human	ELISA	1 x 96 rxns	BTL-LIK-1205
Human ESM-1 (DIYEK C1) ELISA Assay	DIYEK H1	Human Endocan and  CG-cleaved Endocan	To realize one 96 well plate for each biomarker	Human	ELISA Development	1 x 96 rxns (analyze 160 samples)	BTL-LIK-1501
Human ESM-1 (DIYEK C1) ELISA Assay	DIYEK H1	Human Endocan and  CG-cleaved Endocan	To realize five 96 well plate for each biomarker /	Human	ELISA Development	5 x 96 rxns (analyze 400 samples)	BTL-LIK-1501B5
Human ESM-1 (DIYEK C1) ELISA Assay	DIYEK H1	Human Endocan and  CG-cleaved Endocan	To realize ten 96 well plate for each biomarker	Human	ELISA Development	10 x 96 rxns (analyze 800 samples)	BTL-LIK-1501B10
Human ESM-1 (DIYEK C1) ELISA Assay	DIYEK H1	Human Endocan and  CG-cleaved Endocan	To realize twenty-five 96 well plate for each biomarker	Human	ELISA Development	25 x 96 rxns (analyze 2200 samples)	BTL-LIK-1501B25
Ancillary Products for ELISA Development
Human Endocan/ESM-1 Standard	Standard Human Endocan/ESM-1				ELISA Development	100 ng	BTL-LIP-1101
Mouse Endocan/ESM-1 Standard	Standard Murine Endocan/ESM-1				ELISA Development	100 ng	BTL-LIP-1104
Buffer B for Endocan/ESM-1 ELISA (Biothelis)	Buffer B (10X)				ELISA Development	100 ml	BTL-LIP-1201
Buffer C for Endocan/ESM-1 ELISA (Biothelis)	Buffer C (10X)				ELISA Development	10 ml	BTL-LIP-1202
ELISA Buffer for Endocan/ESM-1 ELISA (Biothelis)	ELISA Buffer (20X)				ELISA Development	75 ml	BTL-LIP-1206

 

 All products on this page are for research use only and not for diagnostic or therapeutic use.