Japan Biotherapy Institute (BIJ) : 
BINKIT® for Natural Killer Cell Expansion from Human PBMCs

BINKIT® Features

• Expand Natural Killer (NK) cells from human peripheral blood mononuclear cells (PBMC)
without feeder cells.
• Expand NK cells (CD3-CD56+) several hundred to several thousand-fold in 2 -3 weeks.
• One BINKIT® kit is sufficient to expand NK cells from 20-50 ml of whole blood.

Catalog Number	Product Name	Size
BIJ-N501-1	BINKIT for NK cells expansion from PBMCs	1 Kit
BIJ-N501-2	BINKIT for NK cells expansion from PBMCs	2 Kits
BIJ-N501-4	BINKIT for NK cells expansion from PBMCs	4 Kits
BIJ-N501-8	BINKIT for NK cells expansion from PBMCs	8 Kits
BIJ-N503	BINKIT (T25) for NK cells expansion from PBMCs	1 Kit

Components

• N115a (NK Cell Initial Medium, 45ml) x 1
• N115b (NK Cell Initial Cocktail, 1.9ml) x 1
• N104   (NK Cell Initial Flask,75cm²) x 1
• N201   (NK Cell Subculture Medium, 1000ml) x 1 

Application Data

Ex vivo NK cell expansion using BINKIT

NK cells were expanded from peripheral blood mononuclear cells (PBMCs) using BINKIT®.  CD3-CD56+ cells were enriched from 15.8% to 88.6%

Ex vivo NK cell expansion using BINKIT®

NK cells were expanded using BINKIT® from peripheral blood mononuclear cells (PBMCs) of healthy donors (HD; n=3). Cell count (left) and fold changes (right) indicate the efficient ex vivo expansion of NK cells. 

Ex vivo NK cell expansion using BINKIT®

NK cell cytotoxicity against K562cell line was measured for BINKIT-expanded NK cells (solid lines) and freshly isolated NK cells (dashed lines) from three independent donors.  The expanded NK cells exhibited significantly enhanced cytotoxicity against K562 cell line compared with the freshly isolated NK cells (P < 0.01).  

Key References

• Xuewen Deng, Hiroshi Terunuma, Mie Nieda, Weihua Xiao, Andrew Nicol, Synergistic cytotoxicity of ex vivo expanded natural killer cells in combination with monoclonal antibody drugs against cancer cells, Int Immunopharmacol 14 (2012) 593-605. PMID: 23063974
• Xuewen Deng, Mie Nieda, Hiroshi Terunuma, Ex vivo expanded natural killer cells can possibly kill cancer stem cells, 18th ISCT Annual Meeting 2012.
• Xuewen Deng, Hiroshi Terunuma, Mie Nieda, Cytotoxicity of expanded NK cells against cancer cells is enhanced by monoclonal antibody drugs, 19th ISCT Annual Meeting 2013.