Staining osteoclast marker 'tartrate-resistant acid phosphatase'
TRAP staining kit
The TRAP staining kit can be used to stain the osteoclast marker "Tartrate-Resistant Acid Phosphatase (TRAP)".
Background
Bone mass is controlled by the balance between the activity of bone-forming osteoblasts and the activity of bone-destroying osteoclasts. Osteoblasts have alkaline phosphatase as a marker, whereas osteoclasts have a marker of tartrate-resistant acid phosphatase.
Intended use
- This kit is a product developed as an option for osteoclasts ( human osteoclasts , mouse/rat osteoclasts ).
- Please use it for staining osteoclast marker "tartrate-resistant acid phosphatase".
Composition
- Buffer containing 50 mM tartaric acid (pH 5.0) 50 ml 1 bottle
- Chromogenic substrate 3mg 10 bottles
* Does not include fixative (10% neutral buffered formalin). Please purchase separately or make your own according to the attached manual.
Dyeing example
Figure 1: Examples of TRAP staining
Figure 2: Staining Rat Osteoclasts
Frequently asked questions and answers (FAQ)
Q: Is it possible to quantify after staining?
A: Quantitation is not possible due to the lack of suitable solvents for the extraction of colored pigments.
References
- Morinobu, A., Biao, W., Tanaka, S., Horiuchi, M., Jun, L., Tsuji,G., Sakai, Y., Kurosaka, M., Kumagai, S.
(-)-Epigallocatechin-3-Gallate Suppresses Osteoclast Differentiation and Ameliorates Experimental Arthritis in Mice.
Arthritis Rheum. 58, 2012-2018 (2008), (PMID:18576345) - Hase, H., Kanno, Y., Kojima, H., Sakurai, D., Kobata, T.
Coculture of Osteoclast Precursors With Rheumatoid Synovial Fibroblasts Induces Osteoclastogenesis via Transforming Growth Factor beta-Mediated Down-Regulation of Osteoprotegerin.
Arthritis Rheum. 58, 3356-3365 (2008), (PMID:18975335) - Cao, H., Kuboyama, N.
A Biodegradable Porous Composite Scaffold of PGA/beta-TCP for Bone Tissue Engineering.
Bone. 46, 386-395 (2010), (PMID:19800045) - Okada, Y., Hamada, N., Kim, Y., Takahashi, Y., Sasaguri, K., Ozono, S., Sato, S.
Blockade of Sympathetic beta-receptors Inhibits Porphyromonas Gingivalis-induced Alveolar Bone Loss in an Experimental Rat Periodontitis Model.
Arch. Oral Biol. 55, 502-508 (2010), (PMID:20593554) - Okamoto, A., Ohnishi, T., Bandow, K., Kakimoto, K., Chiba, N., Maeda, A., Fukunaga, T., Miyawaki, S., Matsuguchi, T.
Reduction of Orthodontic Tooth Movement by Experimentally Induced Periodontal Inflammation in Mice.
Eur. J. Oral Sci. 117, 238-247 (2009), (PMID:19583750) - Fujiwara, T., Fukushi, J., Yamamoto, S., Matsumoto, Y., Setsu, N., Oda, Y., Yamada, H., Okada, S., Watari, K., Ono, M., Kuwano, M., Kamura, S., Iida, K., Okada, Y., Koga, M., Iwamoto, Y.
Macrophage Infiltration Predicts a Poor Prognosis for Human Ewing Sarcoma.
Am. J. Pathol. 179, 1157-1170 (2011), (PMID:21771572)
| Catalog Number | Product Name | Size |
| PMC-AK04F-COS | TRAP Staining Kit | 10 Plates |