Real-time PCR primer set (for adipocytes / tissues)

Useful for mouse/rat gene expression analysis

Real-time PCR primer set (for adipocytes/tissues) is a primer set for real-time PCR (Cybergreen method) that is useful for analysis of gene expression in mice and rats

Intended use

The expression level of each gene can be quantitatively measured by real-time PCR (Cybergreen method). It is also designed to have a PCR product length that can be detected by ethidium bromide staining after agarose gel electrophoresis, and can be used in normal RT-PCR.

(Note) The amount of PCR product depends on the sample, PCR reagents, and equipment, so optimization by the customer is required.

Gene primers

part number	Target animal/tissue	Gene primers (gene names are expressed as Official Symbols)
PCRM2	Mouse adipocyte/ adipose tissue	Reference gene (3 types)
		・Actb	・Gapdh	・Hprt
		Target gene (29 types)
		・Adipoq	・Adrb3	・Cebpa	・Cebpb
		・Cebpd	・Fabp4	・Il10	・Il1b
		・Il6	・Insr	・Irs1	・Irs2
		・Lep	・Ccl2	・Serpine 1	・Plin1
		・Pparg	・Rbp4	・Retn	・Slc2a1
		・Slc2a4	・TNF	・Ucp1	・Ucp2
		・Ucp3	・Ppargc1a	・Lipe	・Hsd11b1
		・Prdm16
PCRR1	Rat adipocyte/ adipose tissue	Reference gene (3 types)
		・Actb	・Gapdh	・Hprt1
		Target gene (25 types)
		・Adipoq	・Adrb3	・Cebpa	・Cebpb
		・Cebpd	・Fabp4	・Il10	・Il1b
		・Il6	・Insr	・Irs1	・Irs2
		・Lep	・Ccl2	・Serpine 1	・Plin
		・Pparg	・Rbp4	・Retn	・Slc2a1
		・Slc2a4	・TNF	・Ucp1	・Ucp2
		・Ucp3

Frequently asked questions and answers (FAQ)

Please tell me about the storage method after dissolving.

After dissolving in sterilized water or TE buffer, it is recommended to aliquot and freeze the stock to avoid freezing and thawing as much as possible.

Real-time PCR primer set (for adipocytes/tissues)

Catalog Number	Product Name	Size
PMC-PCRM2	Real Time PCR Primer Set for Adipose - mouse	1 Set
PMC-PCRR1	Real Time PCR Primer Set for Adipose - rat	1 Set