Glucose intracellular uptake measurement kit (wide range, fluorescence method)
Easy measurement of sugar uptake into cells by non-RI method
The enzymatic reaction by glucose-6-phosphate dehydrogenase (G6PDH) produces NADPH in proportion to the amount of 2DG6P, and the fluorescence intensity of the fluorescent substance generated from the fluorescent substrate accompanying the oxidation of NADPH is measured.
Insulin is a hormone that lowers blood sugar, and its action is known to promote sugar transport activity mainly in insulin-sensitive organs such as adipocytes and skeletal muscles, and to take sugar from extracellular to intracellular.
Glucose uptake is generally measured using radiolabeled 3 -O-methyl-D-glucose or 2-deoxyglucose (2DG) such as 3H. Since this method uses radioactive isotopes, it can only be used in RI controlled areas, and there are strict restrictions on its use, so it cannot be used at the normal laboratory level.
This kit is a kit that can easily measure the amount of sugar uptake into cells by a non-RI method that has no safety or regulatory issues.
Fig. 1 Kit measurement principle
2DG taken into the cell is phosphorylated to 2-deoxyglucose-6-phosphate (2DG6P) by hexokinase, but it remains in the cell without proceeding to the next enzymatic reaction.
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Features
| This kit | [Related products] | |
| 2-Deoxyglucose (2DG) metabolic rate measurement kit | ||
| Part number: OKP-PMG-K01 | ||
| Measuring method | Non-RI method | Non-RI method |
| operation time | 3 hours | 5-7 hours (2 days) |
| Detection method | Fluorescence (Ex 540nm/Em 590nm) | Color development (420nm) |
| feature | Rapid measurement over a wide measurement range (0 to 50 μM). | A measurement kit that can quantify with high sensitivity (0 to 5 μM). |
| A one-step method that can also be used for high-throughput assays. |
Composition
This product can measure 100 samples.
- Reaction substrate solution 9 mL × 3 bottles
- 1mM 2DG6P 500μL × 1
- Specimen dilution stock solution 5 mL × 1 bottle
- Fluorescent substrate solution 120 μL × 1 bottle
- Enzyme solution 270 μL × 1 bottle
Things to prepare (Other necessary things)
- Ultrapure water (purified water)
- Fluorescence plate reader (Excitation wavelength 540 nm, Emission wavelength 590 nm)
- 96 well black plate
Example of use
Fig. 2 shows the results of the standard curve, and Fig. 3 shows the results of extracting and measuring 3T3-L1 cells according to the method example and the addition schedule below (*This is an example of measurement).
| Catalog Number | Product Name | Size |
| CSR-MBR-PMG-K01 | Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) | 100 Tests |