Collagen anti-glycation assay kit (Fluorescent AGEs)
Ideal for searching for anti-glycation components
The Collagen Anti-Glycation Assay Kit is a kit that allows you to follow the glycation reaction of collagen in a cell-free and non-enzymatic manner using a 96-well plate. Substances that inhibit the glycation reaction of collagen can be easily screened. Please use it for the development of anti-glycation materials in the development of functional foods and cosmetics.
Background
When sugars coexist with proteins in vivo, they modify and crosslink lysine and arginine residues in proteins, altering the three-dimensional structure of proteins and greatly affecting their activity and physical properties. This reaction is called the glycation reaction (Glycation) or the Maillard reaction. The early reaction produces Amadori rearrangements, and the late reaction leads to advanced glycation endproducts (AGEs) through reactions such as oxidation, dehydration, and condensation. can be divided into Collagen, a structural protein that forms all organs such as skin, blood vessel walls, and bones, is no exception and undergoes glycation.
This product is a kit that can measure the fluorescence (Ex/Em: 370/440 nm) generated from glycated collagen and follow the glycation reaction, making it easy to screen for substances that inhibit the glycation reaction of collagen. increase. Please use it for the development of anti-glycation materials in the development of functional foods and cosmetics.
In recent years, it has been reported that AGEs are produced in vivo not only from glucose, but also from metabolic intermediates and degradation products of glucose, Maillard reaction intermediates, etc. It has been reported that AGEs derived from glyceraldehyde derived from are strongly involved in the onset and progression of diseases.
Features
- All reagents required for the assay are included. ( * The plate is not included in the kit)
- The protocol is simple and can be measured with a fluorescence plate reader (excitation wavelength 370 nm, fluorescence wavelength 440 nm).
- Two 96-well plates can be assayed.
- It is ideal for searching for substances that suppress glycated collagen production.
Product number: AK70 can be used to study the initial saccharification reaction from fructose. Long-term consideration is possible over time.
Product numbers: AK71 and AK72 use glyceraldehyde, which is a sugar metabolism intermediate, to obtain results in a short 24-hour saccharification reaction.
Composition
- [Catalog number AK70]
-
- Neutralized collagen solution
- Glucose solution (200mM)
- Fructose solution (200mM)
- buffer
- Aminoguanidine solution (20 mM) *Anti-glycation standard substance
- [Catalog number AK71]
-
- Neutralized collagen solution
- Glyceraldehyde solution (500 mM)
- buffer
- Aminoguanidine solution (20 mM) *Anti-glycation standard substance
[Catalog number AK72]
- Cartilage type II collagen acidic solution
- neutralizing solution
- Glyceraldehyde solution (500 mM)
- buffer
- Aminoguanidine solution (20 mM) *Anti-glycation standard substance
Kit Comparison
| Catalog number | Product name | Name of sugar/sugar metabolite that initiates saccharification | saccharification period |
| PMC-AK70 | Collagen anti-glycation assay kit | glucose/fructose | Long term (10-30 days) |
| PMC-AK71 | Collagen Anti-Glycation Assay Kit ver.2 | glyceraldehyde | Short time (1 day) |
| PMC-AK72 | Cartilage type II collagen anti-glycation assay kit | glyceraldehyde |
Short time (1 day)
|
Experimental example
Fig. 1 Investigation of glycated collagen production by glycation with glyceraldehyde solution (product number: AK71)
. Aminoguanidine solution was added as a positive control, and hot-water extract from Dogudami leaves and extract of asparagus pseudo-leaf were added as samples, and glycation of each component. The anti-glycation activity of collagen was investigated. As a result, it was confirmed that all of them have concentration-dependent anti-glycation activity.
References
- Rungaroon Waditee-Sirisattha, Hakuto Kageyama.
Protective effects of mycosporine-like amino acid-containing emulsions on UV-treated mouse ear tissue from the viewpoints of antioxidation and antiglycation, Journal of Photochemistry & Photobiology, B: Biology , 223 (2021) 112296
| Catalog Number | Product Name | Size |
| PMC-AK70 | Collagen Glycation Assay Kit, Glucose/Fructose | 1 Kit |
| PMC-AK71 | Collagen Glycation Assay Kit, Glyceraldehyde | 1 Kit |