this page: Ig Purification Dashboard
Product Lineup
Ab-Capcher
Ab-Rapid PuRE
Ab-Rapid SPiN
Ab-Carrier
Manufacturer Website
Protenova Website (External site, Japanese)
Brochures
Ab-Capcher Series Flyer
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High Capacity, Alkaline Resistant, Broad Specificity. Featuring the exclusive R-28A Protein A variant
Ab-Capture: Advanced Tools for Immunoglobulin Purification
Ab-Capcher Products
Ab-Capcher
- IgG Purification Tool Beyond Protein A and G Ab-Capcher™ is ProteoNova's patented Protein A-R28 (Protein A variant) bound at high density to a supporting gel matrix offering many practical advantages for the routine purification of rat and mouse IgG including broader subclass specificity and high capacity binding.
Ab-Carrier
- To deliver antibodies into living cell. It can easily introduce antibodies into living cell efficiently by using function of artificial cell penetrating peptide, controlling cytotoxicity.
Ab-Rapid PuRe
- Ab-Rapid PuRE™ is a specially designed syringe-type column unit prepacked with Ab-Capcher offering maximum convenience for Ig purification.
Application Sheets
Ab-Capcher
No.1_Use of pH 9 buffer with Ab-CapcherTM can greatly increase the yield of mouse monoclonal antibodyNo.2_Ab-Capcher bound 2 to 4 times the mouse IgG1 in ascites as commercial protein A gel and protein G gel
No.3_Ab-Capcher was able to purify 4-fold higher amount of highly purified rat serum IgG than commercially available protein G gel
No.4_Increasing the pH of Ab-Capcher binding buffer increases the yield of rat serum IgG
No.5_Ab-Capcher recovers almost the same amount of human IgG at pH 2.8 as at pH 3.5
No.10a_Ab-Capcher can purify more rat IgG1 from ascites than Protein G
No.10b_Ab-Capcher does not purify rat IgG2a
No.20_Ab-Capcher is an alkaline resistant gel carrier that can be washed with either 0.1N NaOH or 0.5N NaOH for short periods of time
No.23_Ab-Capcher can purify some but not all mouse IgM clones with high yield and high purity
No.29_Ab-Capcher and Ab-Capcher ExTra retain IgG binding capacity of 80% or more than 30 cycles of washing with 0.5N NaOHd
No.30_Ab-Capcher can be used for purification of rat IgG1, IgG2b and IgG2c, but not IgG2a
No.43_Ab-Capcher ExTraTM permits elution at high pH to purify mouse IgG1 from ascites with high yield and purity
No.48_Ab-Capcher ExTra and Protenova Protein L-immobilized gel is effective in combination for purification of Fab fragments
Ab-Carrier
No.25_Ab-Carrier efficiently introduces fluorescently labeled IgG antibodies into Hela cells
No.26_Ab-Carrier introduced anti-NPC antibody into Hela cells and caused impaired proliferation, possibly by inhibiting substance transport across the nuclear membrane
No.27_Ab-Carrier can introduce not only antibodies but also antigen molecules into cells
No.28_Ab-Carrier exhibits minimal cytotoxicity even when added at 5 times the recommended amount
Ab-Rapid PuRe
No.6_Ab-Rapid PuRe column can purify a large amount of high-purity mouse IgG1 from ascites in a short time
No.24_Ab-Rapid PuRe EX yielded 3.8 mg of high purity mouse IgG1 from 2 mL of ascites
No.24_Ab-Rapid PuRe EX yielded 3.8 mg of high purity mouse IgG1 from 2 mL of ascites
Ab-Rapid SPiN
No.15_Ab-Rapid SPiN can purify in 10 minutes 86 percent of the 900 ug of mouse IgG1 contained in 300 uL
No.16_Ab-Rapid SPiN can be used to concentrate and purify even a low-concentration IgG sample by processing the sample in multiple small increments
No.21_Ab-Rapid SPiN can be used to purify IgG from the sera of various animal species with sufficient recovery rate
No.22_Ab-Rapid SPiN can be used to purify IgG from rat serum with almost the same purity and yield as sera from other animal species
No.15_Ab-Rapid SPiN can purify in 10 minutes 86 percent of the 900 ug of mouse IgG1 contained in 300 uL
No.16_Ab-Rapid SPiN can be used to concentrate and purify even a low-concentration IgG sample by processing the sample in multiple small increments
No.21_Ab-Rapid SPiN can be used to purify IgG from the sera of various animal species with sufficient recovery rate
No.22_Ab-Rapid SPiN can be used to purify IgG from rat serum with almost the same purity and yield as sera from other animal species
Mag Beads
No.31_Ab-Capcher Mag purifies more IgG than Protein A Mag Sepharose Xtra and PureProteome Protein A magnetic beads
No.32_Human IgG yield with Ab-Capcher MagTM increased with increasing reaction time
No.33_use Ab-Capcher MagTM in amounts as low as 1_100th the volume of a sample
No.34_Ab-Capcher Mag can be reused
No.35_Ab-Capcher Mag retains most of its IgG-binding ability after washing 30 times with 0.1 N NaOH
No.36_Ab-Capcher Mag yielded manifold the amount of IgG compared to Protein G for mouse and rat
No.31_Ab-Capcher Mag purifies more IgG than Protein A Mag Sepharose Xtra and PureProteome Protein A magnetic beads
No.32_Human IgG yield with Ab-Capcher MagTM increased with increasing reaction time
No.33_use Ab-Capcher MagTM in amounts as low as 1_100th the volume of a sample
No.34_Ab-Capcher Mag can be reused
No.35_Ab-Capcher Mag retains most of its IgG-binding ability after washing 30 times with 0.1 N NaOH
No.36_Ab-Capcher Mag yielded manifold the amount of IgG compared to Protein G for mouse and rat
Ordering Information
| Product Name | Catalog Number | Size | Datasheet |
| Ab-Capcher | PTN-P-002-2 | 2 ml | Datasheet |
| PTN-P-002-10 | 10 ml | ||
| Ab-Capcher ExTra | PTN-P-003-2 | 2 ml | Datasheet |
| PTN-P-003-10 | 10 ml | ||
| Buffer kit for Ab-Rapid Pure | PTN-P-011 | 10 rxns | Datasheet |
| Ab-Rapid Pure 2 | PTN-P-012-2 | 2 Columns | Datasheet |
| Ab-Rapid Pure 10 | PTN-P-012-10 | 10 Columns | |
| Ab-Rapid SPiN10 | PTN-P-013-10 | 10 Pieces | Datasheet |
| Ab-Rapid SPiN50 | PTN-P-013-50 | 50 Pieces | |
| Ab-Rapid SPiN Ex-5mL | PTN-P-014-5-1 | 2 Columns | Datasheet |
| Ab-Rapid SPiN Ex | PTN-P-014-10 | 10 Columns | |
| Ab-Rapid PuRe Ex | PTN-P-015-2 | 2 Columns | Datasheet |
| PTN-P-015-10 | 10 Columns | ||
| Ab-Carrier | PTN-P-101-25 | 25 Tests | Datasheet |
| PTN-P-101-100 | 100 Tests |