Glycobiology

  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-10 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-10)

    Catalog No.(s): CAC-TCS-010

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-9 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-9)

    Catalog No.(s): CAC-TCS-009

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-8 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-8)

    Catalog No.(s): CAC-TCS-008

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-6 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-6)

    Catalog No.(s): CAC-TCS-006

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunostaining of mouse cartilage.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-5)

    Catalog No.(s): CAC-TCS-005

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-3 antibody at 1/500 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-4)

    Catalog No.(s): CAC-TCS-004

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

    Catalog No.(s): CAC-TCS-003

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-2 antibody at 1/100 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-2)

    Catalog No.(s): CAC-TCS-002

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

    Catalog No.(s): CAC-TCS-001

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  • Figure 1. Western blotting of purified human aorta versican (primarily a mixture of V1 and V2 isoforms) resolved by SDS-PAGE on 3-8% linear gradient gels under reducing conditions (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 6B10)

    Catalog No.(s): CAC-PRPG-VS-M04

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  • Figure 1. Western blotting of purified human aorta versican resolved by SDS-PAGE on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 2B3)

    Catalog No.(s): CAC-PRPG-VS-M03

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo-galactosidase-digestion (Digested). The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard). Banding pattern depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, isoforms V0, V1 and V2 do not enter conventional polyacrylamide gels and therefore alternative gel types are strongly recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 4C5)

    Catalog No.(s): CAC-PRPG-VS-M02

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) in its untreated and chondroitinase ABC (Chase ABC)-digested form, or after combined digestion with chondroitinase ABC and a number of exo- and endoglycosydases. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard).

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 5C12)

    Catalog No.(s): CAC-PRPG-VS-M01

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  • Immunostaining of malignant glioblastoma A) and cutaneous melanoma C) lesions. B) Western blot of whole tissue/cell lysates of a human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) Immunostaining of human melanoma MeWo cells. Nuclear counterstaining was performed with Hoescht.

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    Anti NG2 (CSPG4) mAb (Clone 2161D7)

    Catalog No.(s): CAC-PRPG-NG-M01

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  • Figure 1. Western blotting of undigested and endo-beta-galactosidase-digested human cartilage fibromodulin resolved by SDS-PAGE on 7% gels under reducing conditions (band at 60 kDa presumably corresponds to unglycosylated fibromodulin).

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    Anti Fibromodulin (FMOD) mAb (Clone 636B12)

    Catalog No.(s): CAC-PRPG-FBM-M01

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  • Figure 1. Western blotting on intact (left) and chondroitinase ABC-digested (right) bovine decorin after SDS-PAGE on 8% gels.

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    Anti Decorin (DCN) mAb (Clone 889C7)

    Catalog No.(s): CAC-PRPG-DC-M01

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  • Figure 1. Immunoprecipitation of byglycan from human smooth muscle cells and Western blotting with a second anti-biglycan mAb on the intact (right) and chondroitinase ABC predigested (left) precipitate resolved by SDS-PAGE on gradient 4-18% gels.

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    Anti Biglycan (BGN) mAb (Clone 905A7)

    Catalog No.(s): CAC-PRPG-BG-M01

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  • Figure 1. Immunoblotting of a human articular cartilage aggrecan preparation following SDS-PAGE on 3-8% linear gradient gels under reducing conditions, prior to (Intact) and after combined chondroitinase ABC and keratanase II digestion (Digested).

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    Anti Aggrecan (ACAN) mAb (Clone 5G2)

    Catalog No.(s): CAC-PRPG-AG-M03

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  • Figure 1. SDS-PAGE on 3-8% linear gradient gels under reducing conditions of purified human articular cartilage aggrecan prior to (intact) and after combined keratanase I, endo-galactosidase and chondroitinase ABC-digestion.

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    Anti Aggrecan (ACAN) mAb (Clone 5D3)

    Catalog No.(s): CAC-PRPG-AG-M02

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  • Figure 1. Western blotting of purified human cartilage aggrecan. (A) SDS-agarose gel electrophoresis (0.5%) before and after combined chondroitinase ABC digestion of aggrecan. (B) SDS-PAGE on 3-8% linear gradient gels, after the combined digestion of aggrecan indicated in the legend.

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    Anti Aggrecan (ACAN) mAb (Clone 6F4)

    Catalog No.(s): CAC-PRPG-AG-M01

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  • Figure 1. CD44 is a single-pass type I transmembrane protein that functions as a cellular adhesion molecule for hyaluronic acid, a major component of the extracellular matrix. It exists in numerous isoforms that are generated through alternative splicing of CD44 precursor mRNA.

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    Anti CD44 Antigen v9 mAb (Clone RV3)

    Catalog No.(s): CAC-LKG-M003, CAC-LKG-M001

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Please visit our CD44 Variant Antibody homepage to...

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