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(ni-chee-ray-eee)  Venerable Detection Systems for Immunohistochemistry (IHC)

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About Nichirei Biosciences Inc.

Nichirei Biosciences Inc. has developed and improved a patented state-of-the-art Universal Immuno-enzyme Polymer (UIP) technology that it deploys in its N-Histofine® immunohistochemistry (IHC) line of kits. UIP comprises amino acid polymers labeled with multiple molecules of chromogen-producing enzymes [horseradish peroxidase (PO) or alkaline phosphatase (AP)] and secondary antibodies reduced to Fab’ fragments. To eliminate background staining, solid-phase adsorption of secondary antibodies is conducted with relevant sera. N-Histofine® kits are available for human, mouse and rat tissue sections in versions optimized for use with mouse, rabbit, rat, and goat primary antibodies.

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Advantages

1) High sensitivity
2) No background from endogenous biotin
3) Compatible with an IHC Triple-staining method with three different murine primary antibodies [requires Simple Stain™ MAX PO (M) and N-Histofine® Simple Stain™ AP (M)]
4) Compatible with frozen tissue sections (requires N-Histofine® MOUSESTAIN KIT, N-Histofine® Simple Stain™ Mouse MAX PO or N-Histofine® Simple Stain™ Rat MAX PO)
5) Simplified staining steps
6) Ready to use


Product Selection Guide

(Click on the chosen Detection System to link to a detailed description.)

Product_Selection_Guide

Ordering Information 

Product Name
(click for product manual)
Tissue SpeciesCompatible Primary AntibodyCatalog Number
(click for price)
Human Tissue Sections 
N-Histofine Simple Stain MAX PO (MULTI) Human Mouse and Rabbit NIC-414151F
N-Histofine Simple Stain MAX PO (M) Human Mouse NIC-414131F
N-Histofine Simple Stain MAX PO (R) Human Rabbit NIC-414141F
N-Histofine Simple Stain MAX PO (G) Human Goat NIC-414361F
N-Histofine Simple Stain AP (MULTI) Human Mouse and Rabbit NIC-414261F
N-Histofine Simple Stain AP (M) Human Mouse NIC-414241F
N-Histofine Simple Stain AP (R) Human Rabbit NIC-414251F
Mouse Tissue Sections 
N-Histofine MOUSESTAIN KIT Mouse Mouse NIC-414321F
N-Histofine Simple Stain Mouse MAX PO (R) Mouse Rabbit NIC-414341F
N-Histofine Simple Stain Mouse MAX PO (Rat) Mouse Rat NIC-414311F
N-Histofine Simple Stain Mouse MAX PO (G) Mouse Goat NIC-414351F
Rat Tissue Sections 
N-Histofine Simple Stain MAX PO (MULTI) Rat Mouse and Rabbit NIC-414191F
N-Histofine Simple Stain Rat MAX PO (M) Rat Mouse NIC-414171F
N-Histofine Simple Stain Rat MAX PO (R) Rat Rabbit NIC-414181F
N-Histofine Simple Stain Rat MAX PO (G) Rat Goat NIC-414331F
Enzyme Substrate Systems 
N-Histofine DAB-2V All All NIC-425312F
N-Histofine Simple Stain AEC Solution All All NIC-415182F
High_Stain_HRP_(MULTI) Simple_Stain_MAX_PO Simple_Stain_MAX_PO Simple_Stain_MAX_PO Simple_Stain_MAX_PO Simple_Stain_AP Simple_Stain_AP Simple_Stain_AP Simple_Stain_Mouse_MAX_PO Simple_Stain_Mouse_MAX_PO Simple_Stain_Mouse_MAX_PO MOUSESTAIN_KIT Simple_Stain_Rat_MAX_PO Simple_Stain_Rat_MAX_PO Simple_Stain_Rat_MAX_PO Simple_Stain_Rat_MAX_PO