Modified Histone H3/H2B Antibodies

Large line-up of Histone H3 antibodies for ChIP

Monoclonal Antibody Laboratory Co., Ltd. has a large lineup of modified histone antibodies that are ideal for ChIP assays. Nucleosome histone proteins (H2A, H2B, H3, H4) are major components of chromatin. The N-terminus of a histone is called the histone tail and is located a short distance from the nucleosome core. The N-terminal part undergoes various modifications, responds to cell signal stimuli, and undergoes various modifications such as acetylation, phosphorylation, and methylation to affect gene expression. Please make use of the modified histone antibodies that are essential for ChIP assays in epigenetics research from the Cosmo lineup.

Features

All ChIP grade antibodies
Can also be used for Western blotting and immunostaining
Abundant lineup of human modified histone H3 antibodies

Fig. 1 Immunostaining using phosphorylated histone H3 (Ser10) antibody (Product number: MABI0312)

Figure 1. Immunostaining with phosphorylated histone H3 (Ser10) antibody (Product No.: MABI0312)
A: Nuclear staining with DAPI.
B: Staining with phosphorylated histone H3 (Ser10) antibody (product number: MABI0312).
Only the chromosomes of cells in M phase are stained.
C: Composite of A and B.

Applications

Immunoprecipitation: 1-5 μg/5 μl Sepharose
Immunostaining: 0.5-1 μg/ml Sepharose
Western blotting: 0.5-1 μg/ml Sepharose

Histone H3 antibody (unmodified)

Catalog Number	Product Name	Size
MCA-MABI0001-100-EX	Anti Histone H3 mAb (Clone MABI0301(CMA301))	100 ul

Histone H3 antibody K4 (Lysine 4) residue modification

Catalog Number	Product Name	Size
MCA-MABI0002-100-EX	Anti monomethyl Histone H3 (Lys4) mAb (Clone MABI0302)	100 ul
MCA-MABI0003-100-EX	Anti dimethyl Histone H3 (Lys4) mAb (Clone MABI0303)	100 ul
MCA-MABI0004-100-EX	Anti trimethyl Histone H3 (Lys4) mAb (Clone MABI0304)	100 ul

Histone H3 antibody K9 (Lysine 9) residue modification

Catalog Number	Product Name	Size
MCA-MABI0005-100-EX	Anti acetyl Histone H3 (Lys9) mAb (Clone MABI0305)	100 ul
MCA-MABI0006-100-EX	Anti monomethyl Histone H3 (Lys9) mAb (Clone MABI0306)	100 ul
MCA-MABI0007-100-EX	Anti dimethyl Histone H3 (Lys9) mAb (Clone MABI0307)	100 ul
MCA-MABI0008-100-EX	Anti trimethyl Histone H3 (Lys9) mAb (Clone MABI0308)	100 ul

Histone H3 antibody K9/27 (Lysine 9/27) residue modification

Catalog Number	Product Name	Size
MCA-MABI0010-100-EX	Anti acetyl Histone H3 (Lys9/27) mAb (Clone MABI0310)	100 ul

Histone H3 antibody K27 (Lysine 27) residue modification

Catalog Number	Product Name	Size
MCA-MABI0009-100-EX	Anti acetyl Histone H3 (Lys27) mAb (Clone MABI0309)	100 ul
MCA-MABI0321-100-EX	Anti monomethyl Histone H3 (Lys27) mAb (Clone MABI0321)	100 ul
MCA-MABI0323-100-EX	Anti trimethyl Histone H3 (Lys27) mAb (Clone MABI0323)	100 ul
MCA-MABI0324-100-EX	Anti dimethyl Histone H3, dimethyl Lys27 mAb (Clone MABI0324)	100 ul

Histone H3 antibody K36 residue (Lysine 36) modification

Catalog Number	Product Name	Size
MCA-MABI0331-100-EX	Anti monomethyl Histone H3 (Lys36) mAb (Clone MABI0331)	100 ul
MCA-MABI0332-100-EX	Anti dimethyl Histone H3 (Lys36) mAb (Clone MABI0332)	100 ul
MCA-MABI0333-100-EX	Anti trimethyl Histone H3 (Lys36) mAb (Clone MABI0333)	100 ul

Histone H3 antibody S10 (Serine 10) residue modification

Catalog Number	Product Name	Size
MCA-MABI0012-100-EX	Anti phospho Histone H3, Ser10 mAb (Clone MABI0312(CMA312))	100 ul

Histone H2B antibody S14 (Serine 14) residue modification

Catalog Number	Product Name	Size
MCA-MABI0251-100-EX	Anti phospho Histone H2B (Ser14) mAb (Clone MABI0251)	100 ul

CAC Antibody Collection

Epigenetics and chromatin

Epigenetics and chromatin: Histone H3 variants
Product name (click for order info)	Cat No (click for datasheet)	Host	Species specificity
Anti Histone H3.1(HIST1H3A) / H3.2 (HIST1H3B) mAb (Clone 6G3C7)	CAC-CEC-005	RT	HU MS RT MKY HAM
Anti Histone H3.1(HIST1H3A) / H3.2 (HIST1H3B) mAb (Clone 1D4F2)	CAC-CEC-006	MS	HU MS MKY RT HAM CAN
Anti Histone H3.3 (H3F3A) mAb (Clone 6C4A3)	CAC-CEC-007	RT	HU MS RT MKY CAN
Anti Histone H3.3 (H3F3A) mAb (Clone 4H2D7)	CAC-CEC-008	RT	HU MS MKY RT HAM

Epigenetics and chromatin: Post-translationally-modified histone H3
Product name (click for order info)	Cat No (click for datasheet)	Host	Species specificity
Anti Histone H3.1 (HIST1H3A) S10ph mAb (Clone 6G8B7)	CAC-CE-001	RT	HU MS RT MKY HAM
Anti Histone H3.1 (HIST1H3A) T11ph mAb (Clone 6G12C5)	CAC-CE-002	RT	HU MS RT MKY HAM
Anti Histone H3.1 (HIST1H3A) T32ph mAb (Clone 6C7G12)	CAC-CE-003	RT	HU MS RT MKY HAM
Anti Histone H3.1 (HIST1H3A) K9Ac mAb (Clone 2G1F9)	CAC-CE-004	RT	HU MS RT MKY HAM