EVEC (EVC) Dashboard Page

 

Evec's antibodies are fully human antibodies, and of high affinity. Therefore there is no need for either humanization from mouse antibodies, or enhancement of activity by phage display method; they have the property ready to be a pharmaceutical medicine. It is also attractive that tremendous license fees for the antibody developing techniques of humanization or activity enhancement are dispensed with. As mentioned, antibodies developed from human lymphocytes have high affinity because blood lymphocytes are undergone affinity maturation in human body. It was proved to be true in our development of a number of antibodies by EBV method. The affinity of the mouse-based antibodies available in the market is in the range of 10-9M; in contrast, a half of our antibodies have 10-11M.

 

Clone 3-8B. Proliferation inhibition assay with GM-CSF dependent TF-1 (erythroleukemia) cells. IC50 ≥141 ng/ml. IC90 ≥ 5.94 ug/ml.	Clone 1-9F. Proliferation inhibition assay with GM-CSF dependent TF-1 (erythroleukemia) cells. IC50 ≥8.6 ng/ml. IC90 ≥ 183.2 ng/ml.
Clone 1-4G. Cytotoxicityinhibition assay vs IMR-90 (lung fibroblast) cells. IC50 ≥16.4 ng/ml. IC90 ≥ 284 ng/ml.	Clone 4-9A. Cytotoxicity inhibition assay vs IMR-90 (lung fibroblast) cells. IC50 ≥47.2 ng/ml; IC90 ≥ 794.2 ng/ml.

Clone 7-10C. IFFof CHO-K1 cells displaying recombinant HSV gB.

Complement-independent Infectivity inhibition
Virus type	IC50	IC90
HSV-1	0.49 ug/ml	2.72 ug/ml
HSV-2	1.78 ug/ml	16.7 ug/ml

Clone 3-1D IFF of CHO-K1 cells displaying recombinant HSV gD.

Complement-independent Infectivity Inhibition
Virus type	IC50	IC90
HSV-1	284 ng/ml	809 ng/ml
HSV-2	723 ng/ml	2469 ug/ml

Clone S-12C IFF of CHO-K1 cells expressing recombinant MPV-F protein

Complement-independent Infectivity Inhibition
Virus type	IC50
HSV-1	100 ng/ml
HSV-2	300 ng/ml

Neutralizing Recombinant Human Antibodies Derived From Antigen-Screen
EBV-transformed Human Peripheral Mononuclear Cells (produced in CHO-K cells)

Catalog Number	Product Name	Description	Clone	Isotype	Size
EVC-EVHM0101-100	Anti Human Granulocyte Macrophage Colony-Stimulating Factor (hGM-CSF) mAb (Clone 3-8B)	Applications: hGM-CSF activity neutralization, ELISA Protein-A purified (≥95% by SDS-PAGE)	3-8B	IgG1-λ	100 ug
EVC-EVHM0102-100	Anti Granulocyte Macrophage Colony-Stimulating Factor mAb (Clone 1-9F)		1-9F	IgG1-λ	100 ug
EVC-EVHM0301-100	Anti HSV Type 1 + Type 2 Glycoprotein B (HSV-gB) mAb (Clone 7-10C)	Applications: ICC/IF, Infectivity neutralization Protein-A purified (≥95% by SDS-PAGE)	7-10C	IgG1-λ	100 ug
EVC-EVHM0401-100	Anti HSV Type 1 + 2 Glycoprotein D (HSV-gD) mAb (Clone 3-1D)	Applications: ICC/IF, Infectivity neutralization Protein-A purified (≥95% by SDS-PAGE)	3-1D	IgG1-κ	100 ug
EVC-EVHM0501-100	Anti Metapneumovirus F Glycoprotein (Protein F) mAb (Clone 5-12C)	Applications: ICC/IF, FCM, infectivity neutralization Protein-A purified (≥95% by SDS-PAGE)	5-12C	IgG1-λ	100 ug
EVC-EVHM0601-100	Anti Clostridium difficile Toxin A mAb (Clone 1-4G)	Applications: FCM, infectivity neutralization, ELISA Protein-A purified (≥95% by SDS-PAGE)	1-4G	IgG1-λ	100 ug
EVC-EVHM0701-100	Anti Clostridium difficile Toxin B mAb (Clone 4-9A)		4-9A	IgG1-λ	100 ug
EVC-EVHM0201-100	Anti Cytomegalovirus Glycoprotein B mAb (Clone 5-7D)	Applications: Infectivity neutralization; FCM,  ELISA, WB Protein-A purified (≥95% by SDS-PAGE)	5-7D	IgG1-κ	100 ug

Human IgG1 Control mAb

Fully human monoclonal antibody vs antigen not present in mammalian cells. Useful as negative control antibody in bioassays and in vivo studies.

Catalog Number	Product Name	Clone	Isotype	Size
EVC-EVHM-C02-100	IgG1 (KAPPA) mAb	9-10E	IgG1-κ	100 ug
EVC-EVHM-C03-100	IgG1 (LAMBDA) mAb)	1-3E	IgG1-λ	100 ug

Human Antibodies

These human recombinant antibodies are high affinity monoclonals with heavy and light chains cloned from antigen screened EBV-transformed human peripheral blood B lymphocytes.
Peripheral blood lymphocytes provide a pool of activated high binding antibody-producing cells subjected to natural affinity maturation from repeated exposure to low antigen concentrations in the human body.

Catalog Number	Product Name	Description	Clone	Antibody Isotype	Size	Datasheet
EVC-EVHM0101-100	Anti Granulocyte Macrophage Colony-Stimulating Factor mAb (Clone 3-8B))	Fully recombinant human IgG1-lambda mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE). Applications: Antigen-neutralization; ELISA (others not tested).	3-8B	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0102-100	Anti Granulocyte Macrophage Colony-Stimulating Factor mAb (Clone 1-9F)		1-9F	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0301-100	Anti HSV Envelope Glycoprotein B (gB) mAb (Clone 7-10C)	Fully recombinant human IgG1-lambda mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE). Applications: Antigen-neutralization; ELISA (others not tested).Applications: ICC/IF, Neutralization assay (others not tested)	7-10C	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0401-100	Anti HSV Envelope Glycoprotein D mAb (Clone 3-1D)	Fully recombinant human IgG1-Kappa mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE). Applications: Antigen-neutralization; ELISA (others not tested).Applications: ICC/IF, Neutralization assay (others not tested)	3-1D	IgG1-kappa	100 ug	Datasheet
EVC-EVHM0501-100	Anti Metapneumovirus Fusion Glycoprotein F0 (Protein F) mAb (Clone 5-12C)	Fully recombinant human IgG1-Lambda mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE).  Applications: ICC/IF, FCM, Neutralization assay (Others not tested)	5-12C	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0601-100	Anti Clostridium difficile Toxin A mAb (Clone 1-4G)	Fully recombinant human IgG1-Lambda mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE).  Applications: ELISA, FCM, Neutralization assay (Others not tested)	1-4G	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0701-100	Anti Clostridium difficile Toxin B mAb (Clone 4-9A)		4-9A	IgG1-lambda	100 ug	Datasheet
EVC-EVHM0201-100	Anti Cytomegalovirus Glycoprotein B mAb (Clone 5-7D)	Fully recombinant human IgG1-Kappa mAb with H+L chain genes cloned from EBV transformed human peripheral monocnuclear cells screened for antigen reactivity. Produced in CHO-K cells. Protein-A purified (>95% by SDS-PAGE).  Applications: ELISA, Western Blot, FCM, Neutralization assay.	5-7D	IgG1-kappa	100 ug	Datasheet

Other Antibodies

Catalog Number	Product Name	Clone	Antibody Isotype	Size	Datasheet
EVC-EVHM-C02-100	IgG1 (KAPPA) mAb (Clone 9-10E)	9-10E	IgG1-kappa	100 ug	Datasheet
EVC-EVHM-C03-100	IgG1 (LAMBDA) mAb (Clone 1-3E)	1-3E	IgG1-lambda	100 ug	Datasheet

Method of Antibody Production